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NOS1 (Y846) polyclonal antibody（KU）

货号: BS1821

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1821

Product Name :NOS1 (Y846) polyclonal antibody（KU）

Swiss-Prot :P29475

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NOS1 (Y846) polyclonal antibody detects endogenous levels of NOS1 protein.

BiowMW :~ 160 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Nitric oxide synthase, brain; Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; N-NOS; nNOS; Peptidyl-cysteine S-nitrosylase NOS1; bNOS; NOS1

Immunogen :Synthetic peptide, corresponding to amino acids 815-862 of Human NOS1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NOS1 (Y846) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(20ug) Lane4:C6 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of NOS1 (Y846) pAb in paraffin-embedded human lung carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of NOS1 (Y846) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50.showing cytoplasmic and cell membrane staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Chlorogenic acid alleviate kidney fibrosis through regulating TLR4/NF-қB mediated oxidative stress and inflammation

JournalJOURNAL OF ETHNOPHARMACOLOGY

ApplicationWB

IF4.8

SpeciesMouse,Human

PMID39142620