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PDCD4 (K453) polyclonal antibody（KU）

货号: BS1805

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1805

Product Name :PDCD4 (K453) polyclonal antibody（KU）

Swiss-Prot :Q53EL6

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The transformation suppressor gene Pdcd-4 (programmed cell death gene 4) inhibits the tumor-promoter mediated transformation of mouse keratinocytes and is a potential tumor suppressor gene in the development of human lung cancer. Biochemical analysis suggests that the Pdcd-4 protein is involved in protein translation as well as in nuclear events. Pdcd-4 directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Pdcd-4 also suppresses the transactivation of AP-1 responsive promoters by c-Jun, suggesting that the transformation-suppressor activity of Pdcd-4 might be due, at least in part, to the inhibition of c-Jun activity. In addition to affecting c-Jun phosphorylation, Pdcd-4 blocks the recruitment of the co-activator p300 by c-Jun.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PDCD4 (K453) polyclonal antibody detects endogenous levels of PDCD4 protein.

BiowMW :~ 51 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral elF4 and p70 S6 Kinase,

Alternative Name :Programmed cell death protein 4; Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; PDCD4; H731

Immunogen :Synthetic peptide, corresponding to amino acids 422-474 of Human PDCD4.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PDCD4 (K453) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:CT26 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PDCD4 (K453) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.