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Separase (A795) polyclonal antibody

货号: BS1710

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1710

Product Name :Separase (A795) polyclonal antibody

Swiss-Prot :Q14674

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Separase is a cysteine protease that triggers anaphase in all eukaryotes by participating in separation of sister chromatids during mitosis. Once activated, Separase hydrolyzes the SCC1 subunit of cohesin, the chromosomal protein complex responsible for sister chromatid cohesion. Separase and cohesin are highly conserved from yeasts to humans. When the cell is not dividing, Separase is prevented from cleaving cohesin through its association with another protein, securin. When anaphase is signaled, the securin is ubiquitinated and hydrolyzed by APC/cyclosome, releasing the active Separase. Separase is transiently activated between the two meioses and may also be involved in homolog separation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Separase (A795) polyclonal antibody detects endogenous levels of Separase protein.

BiowMW :~ 230 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Separin; Caspase-like protein ESPL1; Extra spindle poles-like 1 protein; Separase; ESPL1; ESP1; KIAA0165

Immunogen :Synthetic peptide, corresponding to amino acids 763-824 of Human Separase.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Separase (A795) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate

Immunohistochemistry (IHC) analyzes of Separase (A795) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.