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SHP-2 (E574) polyclonal antibody（KU）

货号: BS1705

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1705

Product Name :SHP-2 (E574) polyclonal antibody（KU）

Swiss-Prot :Q06124

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SHP-2 (E574) polyclonal antibody detects endogenous levels of SHP-2 protein.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Immune Cell Signaling,Insulin &Glucose Signaling,B cell receptor signaling,

Alternative Name :Tyrosine-protein phosphatase non-receptor type 11; Protein-tyrosine phosphatase 1D; PTP-1D; Protein-tyrosine phosphatase 2C; PTP-2C; SH-PTP2; SHP-2; Shp2; SH-PTP3; PTPN11; PTP2C; SHPTP2

Immunogen :Synthetic peptide, corresponding to amino acids 550-600 of Human SHP-2.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of SHP-2 (E574) polyclonal antibody at 1:500 dilution Lane1:H9C2 whole cell lysate(40ug) Lane2:SP2/0 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of SHP-2 (E574) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of L02 cell lysate using SHP-2 (E574) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using SHP-2 (E574) pAb #BS1705.“↑”（supernatant）; “↓”（deposition）

The tumor suppressor role of Src homology phosphotyrosine phosphatase 2 in hepatocellular carcinoma

JournalJournal Of Cancer Research And Clinical Oncology

ApplicationIHC

IF4.553

SpeciesHuman

PMID22228034
Tumorigenic role of YAP in hepatocellular carcinogenesis is involved in SHP2 whose function is different in vitro and in vivo

JournalPathology Research And Practice

ApplicationWB,IHC

IF3.25

SpeciesHuman

PMID29699904