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YAP (H122) polyclonal antibody

货号: BS1701

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1701

Product Name :YAP (H122) polyclonal antibody

Swiss-Prot :P46937

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :YAP (H122) polyclonal antibody detects endogenous levels of YAP protein.

BiowMW :~ 38, 67 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :hippo Signaling,

Alternative Name :Yorkie homolog; 65 kDa Yes-associated protein; YAP1; YAP65

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human YAP.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of YAP (H122) polyclonal antibody in extracts from HepG2 cells.

Immunohistochemistry (IHC) analyzes of YAP (H122) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

L1CAM–ILK-YAP Mechanotransduction Drives Proliferative Activity of Epithelial Cells in Middle Ear Cholesteatoma

JournalAmerican Journal Of Pathology

ApplicationIHC

IF3.491

SpeciesRat,Mouse

PMID32360569
miR‑497 inhibits proliferation and invasion in triple‑negative breast cancer cells via YAP1

JournalOncology Letters

ApplicationWB

IF2.967

SpeciesHuman

PMID34122631