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VCL polyclonal antibody（KU）

货号: BS1700

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1700

Product Name :VCL polyclonal antibody（KU）

Swiss-Prot :P18206

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development. Three structural vinculin domains include an amino-terminal head, a short, flexible proline-rich region and a carboxy-terminal tail . In the inactive state, the head and tail domains of vinculin interact to form a closed confirmation. The open and active form of vinculin translocates to focal adhesions where it is thought to be involved in anchoring F-actin to the membrane and regulation of cell migration. Phospholipid binding to the tail domain and subsequent phosphorylation of vinculin at Ser1033 and Ser1045 by PKC-α and Tyr100 and Tyr1065 by Src kinases weakens the head-tail interaction. This change in vinculin allows the binding of a number of other proteins, including talin, α-actinin and paxillin, which disrupts the head-tail interaction and initiates the conformational change from the inactive to active state. Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth. This is supported by a demonstrated relationship between decreased vinculin expression and increased carcinogenesis and metastasis in colorectal carcinoma.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :VCL polyclonal antibody detects endogenous levels of VCL protein.

BiowMW :~ 130kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,

Alternative Name :Vinculin;Metavinculin;MV;VCL

Immunogen :Synthetic peptide, corresponding to Human VCL.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of VCL polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:CT-26 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:PC12 whole cell lysate(40ug) Lane6:HepG2 whole cell lysate(40ug) Lane7:Hela whole cell lysate(40ug)