产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Skp1 p19 (P72) polyclonal antibody

货号: BS1694

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1694

Product Name :Skp1 p19 (P72) polyclonal antibody

Swiss-Prot :P63208

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Skp1 p19 (P72) polyclonal antibody detects endogenous levels of Skp1 p19 protein.

BiowMW :~ 19 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :p19A; RNA polymerase II elongation factor-like protein; Organ of Corti protein 2; OCP-2; OCP-II; Transcription elongation factor B; SIII; SKP1; EMC19; OCP2; SKP1A; TCEB1L

Immunogen :Synthetic peptide, corresponding to amino acids 40-90 of Human Skp1 p19.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SKP1 pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A2780 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Skp1 p19 (P72) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.