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NIFK (D229) polyclonal antibody

货号: BS1679

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1679

Product Name :NIFK (D229) polyclonal antibody

Swiss-Prot :Q9BYG3

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The structural proteins for the complex metalloenzyme nitrogenase include NIFK, NIFD and NIFH. These proteins are all necessary for archaeal and bacterial nitrogen fixation. The NIFK gene encodes the b subunit of the nitrogenase molybdenum-iron (MoFe) tetramer. NIFK localizes to the nucleolus where it interactes with the fork-head associated domain of the proliferation marker protein Ki-67 in a mitosis-specific and phosphorylation-dependent manner. NIFK is widely expressed in adult tissues, suggesting other functions in addition to its interaction with Ki-67, which is only expressed in proliferating cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NIFK (D229) polyclonal antibody detects endogenous levels of NIFK protein.

BiowMW :~ 34 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MKI67 FHA domain-interacting nucleolar phosphoprotein; Nucleolar phosphoprotein Nopp34; Nucleolar protein interacting with the FHA domain of pKI-67; hNIFK; NIFK; MKI67IP; NOPP34

Immunogen :Synthetic peptide, corresponding to amino acids 196-245 of Human NIFK.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NIFK (D229) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Immunohistochemistry (IHC) analyzes of NIFK (D229) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.