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Sp1 (G447) polyclonal antibody（KU）

货号: BS1598

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1598

Product Name :Sp1 (G447) polyclonal antibody（KU）

Swiss-Prot :P08047

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 was initially identified as a HeLa cell-derived factor that selectively activates in vitro transcription from the SV40 promoter and binds to the multiple GC boxes in the 21 bp repeated elements in SV40. The sequence specificity of DNA binding is conferred by Zn (II) fingers, whereas a different region of Sp1 appears to regulate the affinity of DNA binding. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SP1 (G447) polyclonal antibody detects endogenous levels of SP1 protein.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :ESC Pluripotency and Differentiation Signaling Pathway,

Alternative Name :Transcription factor Sp1; SP1; TSFP1

Immunogen :Synthetic peptide, corresponding to amino acids 421-470 of Human Sp1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Sp1 (G447) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:CT26 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:COS-7 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Sp1 (G447) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

The TGF‐β‐induced up‐regulation of NKG2DLs requires AKT/GSK‐3β‐mediated stabilization of SP1

JournalJournal Of Cellular And Molecular Medicine

ApplicationWB,IP,IF

IF4.486

SpeciesHuman

PMID28165192
Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues

JournalInternational Journal of Ophthalmology

ApplicationWB

IF1.33

SpeciesHuman

PMID33614450
Expression of specificity protein 1 and collagen I in primary pterygial tissues

JournalArquivos Brasileiros De Oftalmologia

ApplicationWB

IF0.859

SpeciesHuman

PMID31166424