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RPS6 (L234) polyclonal antibody（KU）

货号: BS1592

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1592

Product Name :RPS6 (L234) polyclonal antibody（KU）

Swiss-Prot :P62753

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB IP

Application_all :WB: 1:5000~1:10000 IP: 1:50~1:200

Background :One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :RPS6 (L234) polyclonal antibody detects endogenous levels of RPS6 protein.

BiowMW :~ 32 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :MAPK Erk in Growth and Differentiation Pathway(,PI3K AKT signaling,Translational Contral elF4 and p70 S6 Kinase,

Alternative Name :40S ribosomal protein S6; Phosphoprotein NP33; Small ribosomal subunit protein Es6; RPS6; OK/SW-cl.2

Immunogen :Synthetic peptide, corresponding to Human RPS6.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of RPS6 (L234) pAb at 1:5000 dilution Lane1:AML-12 whole cell lysate(40ug) Lane2:The Liver tissue lysate of Mouse(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:Jurket whole cell lysate(40ug) Lane5:THP-1 whole cell lysate(40ug)

Immunoprecipitation of HEK293T cell lysates using RPS6 pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using RPS6 pAb #BS1592.

Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression

JournalMolecular Medicine

ApplicationWB

IF4.096

SpeciesHuman

PMID33109073