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Rabphilin-3A (P231) polyclonal antibody（KU）

货号: BS1590

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1590

Product Name :Rabphilin-3A (P231) polyclonal antibody（KU）

Swiss-Prot :P47708

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B,and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Rabphilin 3A (P231) polyclonal antibody detects endogenous levels of Rabphilin 3A protein

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Rabphilin-3A; Exophilin-1; Exophilin 1; Exophilin1; Rph3a

Immunogen :Synthetic peptide, corresponding to amino acids 200-250 of Human Rabphilin-3A.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Rabphilin-3A (P231) polyclonal antibody at 1:500 dilution Lane1:THP-1 whole cell lysate Lane2:NIH-3T3 whole cell lysate Lane3:H9C2 whole cell lysate

Immunohistochemistry (IHC) analyzes of Rabphilin-3A (P231) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.