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PKC ζ (P556) polyclonal antibody（KU）

货号: BS1579

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1579

Product Name :PKC ζ (P556) polyclonal antibody（KU）

Swiss-Prot :Q05513

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP IF

Application_all :WB: 1:500~1:1000 IHC/IF: 1:50~1:200 IP: 1:50~1:200

Background :Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (α, βI, Βii and γ) and novel (n) PKC isoforms (δ, ε , ζ, η and θ). Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of nPKC δ and ε are independent of Ca++. On the other hand, nPKC δ and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PKC ζ (P556) polyclonal antibody detects endogenous levels of PKC ζ protein.

BiowMW :~ 72 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein kinase C zeta type; nPKC-zeta; PRKCZ; PKC2; PKC zeta

Immunogen :Synthetic peptide, corresponding to amino acids 520-570 of Human PKC ζ.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PKC ζ (P556) polyclonal antibody at 1:2000 dilution Lane1:The Testis tissue lysate of Mouse(40ug) Lane2:PC12 cell lysate Lane3:A549 cell lysate Lane4:U-87MG cell lysate

Immunohistochemistry (IHC) analyzes of PKC ζ (P556) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Immunoprecipitation of the Lung tissue lysate of Mouse using PKC ζ (P556) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using PKC ζ (P556) pAb #BS1579.“↑”(supernatant); “↓”(deposition)

Norisoboldine inhibits the production of interleukin-6 in fibroblast-like synoviocytes from adjuvant arthritis rats through PKC/MAPK/NF-κB-p65/CREB pathways

JournalJournal Of Cellular Biochemistry

ApplicationWB

IF4.429

SpeciesRat

PMID22473817
HGF-Induced PKCζ Activation Increases Functional CXCR4 Expression in Human Breast Cancer Cells

JournalPLoS One

ApplicationWB,IHC

IF2.74

SpeciesHuman,Mouse

PMID22242160