产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PBK (I4) polyclonal antibody（KU）

货号: BS1571

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1571

Product Name :PBK (I4) polyclonal antibody（KU）

Swiss-Prot :Q96KB5

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :PBK (PDZ binding kinase) belongs to the serine/threonine protein kinase family. It is related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family and phosphorylates MAP kinase p38. It is active only in mitosis. When phosphorylated it forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage. It makes a kinase-substrate complex with cdk1/cyclin B1 and PRC1 on microtubules during mitosis, enhances the cdk1/cyclin B1-dependent phosphorylation of PRC1 and thereby strongly promotes cytokinesis. It may play a role in the activation of lymphoid cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PBK/TOPK (I4) polyclonal antibody detects endogenous levels of PBK/TOPK protein

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Lymphokine-activated killer T-cell-originated protein kinase; Cancer/testis antigen 84; CT84; MAPKK-like protein kinase; Nori-3; PDZ-binding kinase; Spermatogenesis-related protein kinase; SPK; T-LAK cell-originated protein kinase; PBK; TOPK

Immunogen :Synthetic peptide, corresponding to the N-terminal of Human PBK.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PBK (I4) pAb at 1:500 dilution Lane1:PMVEC whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:3T3-L1 whole cell lysate(40ug) Lane4:HuT78 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PBK (I4) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.