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PAK1/2/3 (E417) polyclonal antibody(KU)

货号: BS1570
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS1570
Product Name :PAK1/2/3 (E417) polyclonal antibody(KU)
Swiss-Prot :Q13153/Q13177/O75914
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB IF
Application_all :WB: 1:1000~1:2000 IF: 1:50~1:200
Background :Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :PAK1/2/3 (E417) polyclonal antibody detects endogenous levels of total PAK protein.
BiowMW :~ 61 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Regulation of Actin Dynamics,ErbB HER Signaling,MAPK Erk in Growth and Differentiation Pathway(,Regulation of Microtubule Dynamics,TGFβ Smad signaling,
Alternative Name :Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; PAK1; PAKα; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK-2p27; PAK-2p34; C-t-PAK2; PAK2; PAKγ; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3; PAK3; OPHN3; PAKβ;
Immunogen :Synthetic peptide, corresponding to Human PAK1/2/3.
Conjugate :Unconjugated
Modification :Unmodification
Western blot (WB) analysis of PAK1/2/3 (E417) polyclonal antibody at 1:1000 dilution Lane1:SSGC7901 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:THP-1 whole cell lysate(40ug) Lane4:RAW264.7 whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug)
Immunofluorescence analysis of U-87MG cells using PAK1/2/3 (E417) antibody at dilution of 1:50.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com