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p40-phox (R149) polyclonal antibody（KU）

货号: BS1563

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1563

Product Name :p40-phox (R149) polyclonal antibody（KU）

Swiss-Prot :Q15080

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase is a multimeric enzyme system that mediates electron transport from NADPH in the cytoplasm to molecular oxygen in the phagosome, thereby generating reactive oxidant intermediates. Upon neutrophil stimulation, NADPH-oxidase and other cytosolic elements localize to the cell membrane from the cytosol to form a complex which produces phagocytic oxygen radicals. There are a number of cytosolic proteins that are involved in NADPH-oxidase activation/deactivation,including p47-phox, p67-phox, p40-phox and the small GTP-binding protein, Rac. Activation of NADPH oxidase is accompanied by the phosphorylation of cytosolic components p40-phox, p47-phox and p67-phox. The PKC consensus phosphorylation sites Thr 154 and Ser 315 in p40-phox are phosphorylated during activation of NADPH oxidase. p40-phox can promote oxidase activation by increasing the affinity of p47-phox for NADPH-oxidase.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :p40 phox (R149) polyclonal antibody detects endogenous levels of p40 phox protein.

BiowMW :~ 39 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Neutrophil cytosol factor 4; NCF-4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40phox; NCF4; SH3PXD4

Immunogen :Synthetic peptide, corresponding to amino acids 115-170 of Human p40-phox.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of p40-phox (R149) pAb at 1:500 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:C6 whole cell lysate(40ug) Lane4:Jurkat whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of p40-phox (R149) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Arctigenin suppresses transforming growth factor-β1-induced expression of monocyte chemoattractant protein-1 and the subsequent epithelial–mesenchymal transition through reactive oxygen species-dependent ERK/NF-κB signaling pathway in renal tubular epithe

JournalFree Radical Research

ApplicationWB

IF2.825

SpeciesHuman

PMID25968940
Testosterone improves erectile function through inhibition of reactive oxygen species generation in castrated rats

JournalPeerJ

ApplicationWB

IF2.379

SpeciesRat

PMID27168996