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IRS-1 (L789) polyclonal antibody（KU）

货号: BS1543

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1543

Product Name :IRS-1 (L789) polyclonal antibody（KU）

Swiss-Prot :P35568

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF

Application_all :WB: 1:2000~1:5000 IF: 1:50~1:200

Background :Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IRS-1 (L789) polyclonal antibody detects endogenous levels of IRS-1 protein.

BiowMW :~ 175 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,MTOR signaling,

Alternative Name :Insulin receptor substrate 1; IRS-1; IRS1

Immunogen :Synthetic peptide, corresponding to Human IRS-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of IRS-1 (L789) polyclonal antibody at 1:2000 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:BV2 whole cell lysate(40ug) Lane4:PMVEC whole cell lysate(40ug)

Immunofluorescence analysis of MCF-7 cells using IRS-1 (L789) antibody at dilution of 1:50.