Product Name :PRAS40 (P240) polyclonal antibody（KU）
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:500~1:1000
IHC: 1:50~1:200
Background :Akt, also known as protein kinase B, is one of the major downstream targets of the phosphatidylinositol 3-kinase pathway. This protein kinase has been implicated in insulin signaling, stimulation of cellular growth, inhibition of apoptosis and transformation of cells. The proline-rich Akt substrate PRAS40, also designated AKT1S1, becomes phosphorylated by activated Akt on Ser or Thr residues in the motif RXRXX(S/T). Phosphorylated PRAS40 subsequently binds 14-3-3 in a sequence-specific manner, thereby inducing such changes as alteration of protein subcellular localization and regulation of intrinsic enzymatic activity. Studies also suggest that PRAS40 phosphorylation and its interaction with pAkt and 14-3-3 may play an important role in neuroprotection mediated by NGF in apoptotic neuronal cell death after cerebral ischemia. PRAS40 maps to human chromosome 19q13.33.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Akt1 S1 (P240) polyclonal antibody detects endogenous levels of Akt1 S1 protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :PI3K AKT signaling,Translational Contral elF4 and p70 S6 Kinase,Insulin &Glucose Signaling,MTOR signaling,
Alternative Name :Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate; AKT1S1; PRAS40
Immunogen :Synthetic peptide, corresponding to amino acids 210-260 of Human PRAS40.
Modification :Unmodification
