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Topo IIβ (A32) polyclonal antibody（KU）

货号: BS1479

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1479

Product Name :Topo IIβ (A32) polyclonal antibody（KU）

Swiss-Prot :Q02880

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Topoisomerase II β (A32) polyclonal antibody detects endogenous levels of Topoisomerase II β protein.

BiowMW :~ 183 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TOP2B; DNA topoisomerase 2-beta; DNA topoisomerase II, beta isozyme

Immunogen :Synthetic peptide, corresponding to the N-terminus of Human Topo IIβ.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Topo IIβ (A32) pAb at 1:500 dilution Lane1:A549 whole cell lysate(20ug) Lane2:HEK293T whole cell lysate(20ug) Lane3:AML-12 whole cell lysate(20ug) Lane4:PC12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Topo IIβ (A32) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.

Tetramethylpyrazine promotes SH-SY5Y cell differentiation into neurons through epigenetic regulation of Topoisomerase IIβ

JournalNeuroscience

ApplicationWB

IF3.59

SpeciesHuman

PMID25149630
Tetramethylpyrazine induces SH-SY5Y cell differentiation toward the neuronal phenotype through activation of the PI3K/Akt/Sp1/TopoIIβ pathway

JournalEuropean Journal Of Cell Biology

ApplicationWB

IF3.025

SpeciesHuman

PMID26518113
13-Cis Retinoic Acid Induces Neuronal Differentiation in Daoy (Medulloblastoma) Cells Through Epigenetic Regulation of Topoisomerase IIβ

JournalAPPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

ApplicationWB

IF3.094

SpeciesHuman

PMID37000354