产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PIASy (D488) polyclonal antibody（KU）

货号: BS1468

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1468

Product Name :PIASy (D488) polyclonal antibody（KU）

Swiss-Prot :Q8N2W9

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PIAS4 (D488) polyclonal antibody detects endogenous levels of PIAS4 protein.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Jak Stat ：IL-6 Receptor Signaling,

Alternative Name :E3 SUMO-protein ligase PIAS4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma; PIASy; PIAS4; PIASG

Immunogen :Synthetic peptide, corresponding to amino acids 455-506 of Human PIASy.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PIASy (D488) pAb at 1:1000 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:MEF whole cell lysate(40ug) Lane3:The Testis tissue lysate of Rat(20ug) Lane4:HEK293T whole cell lysate(10ug) Lane5:K562 whole cell lysate(10ug)

Immunohistochemistry (IHC) analyzes of PIASy (D488) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.