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PIAS3 (F25) polyclonal antibody（KU）

货号: BS1467

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1467

Product Name :PIAS3 (F25) polyclonal antibody（KU）

Swiss-Prot :Q9Y6X2

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IF

Application_all :WB: 1:5000~1:10000 IF: 1:50~1:200

Background :The IL-6 type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization, and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PIAS3 (F25) polyclonal antibody detects endogenous levels of PIAS3 protein.

BiowMW :~ 75 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Jak Stat ：IL-6 Receptor Signaling,

Alternative Name :E3 SUMO-protein ligase PIAS1; Protein inhibitor of activated STAT protein 1; Gu binding protein; GBP; RNA helicase II binding protein; DEAD/H box-binding protein 1; DDXBP1

Immunogen :Synthetic peptide, corresponding to Human PIAS3.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PIAS3 (F25) polyclonal antibody at 1:5000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HeK293T whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:MEF whole cell lysate(40ug) Lane6:The Testis tissue lysate of Mouse(40ug) Lane7:The Brain tissue lysate of Rat(40ug)

Immunofluorescence analysis of Hela cells using PIAS3 antibody at dilution of 1:50.

Correlative analyses of the expression levels of PIAS3, p-SHP2, SOCS1 and SOCS3 with STAT3 activation in human astrocytomas

JournalMolecular Medicine Reports

ApplicationIHC

IF2.952

SpeciesHuman

PMID28035384