产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

NUB1 (Y601) polyclonal antibody（KU）

货号: BS1464

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1464

Product Name :NUB1 (Y601) polyclonal antibody（KU）

Swiss-Prot :Q9Y5A7

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:10~1:100

Background :NEDD8 is a ubiquitin-like protein that controls vital biological events through its conjugation to cullin proteins. NEDD8 ultimate buster-1 (NUB1), is a negative regulator of the NEDD8 system that recruits NEDD8 and its conjugates to the proteasome for degradation. It is, therefore a cell growth regulator. The UBA domain of NUB1 is the specific acceptor for the linear ubiquitin precursor. NUB1 is composed of 601 amino acids. It is an interferon-inducible protein and predominantly localizes in the nucleus. NUB1 is specifically expressed in adult human testis, ovary, heart, and skeletal muscle tissues.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NUB1 (Y601) polyclonal antibody detects endogenous levels of NUB1 protein.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NEDD8 ultimate buster 1; Negative regulator of ubiquitin-like proteins 1; Renal carcinoma antigen NY-REN-18; NUB1; NYREN18

Immunogen :Synthetic peptide, corresponding to amino acids 570-621 of Human NUB1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NUB1 (Y601) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(10ug) Lane2:A549 whole cell lysate(10ug) Lane3:U-87MG whole cell lysate(10ug) Lane4:MCF-7 whole cell lysate(10ug) Lane5:PC3 whole cell lysate(10ug) Lane6:PC12 whole cell lysate(40ug) Lane7:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of NUB1 (Y601) pAb in paraffin-embedded human tonsil tissue.

Immunoprecipitation of HEK293T cell lysate using NUB1 (Y601) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) .Lane 1 is 30% input. The western blot was probed using NUB1 (Y601) #BS1464.“↑”（supernatant）; “↓(deposition）