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c-PLA2 (N499) polyclonal antibody（KU）

货号: BS1434

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1434

Product Name :c-PLA2 (N499) polyclonal antibody（KU）

Swiss-Prot :P47712

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Phospholipase A2s (PLA2s) constitute a family of esterases that hydrolyze the sn-2-acyl ester bond in glycerophospholipid molecules. These enzymes are generally calcium-dependent and have been found both intra- and extracellularly. By hydrolyzing the sn-2 bond in glycerophospholipids, PLA2s release fatty acids. One such fatty acid, arachidonic acid, generates the substrates for the initiation of the arachidonic acid cascade that produces various eicosanoids (i.e. prostaglandins, leukotrienes and thromboxanes), many of which are potent mediators of inflammation. PLA2s include both the relatively low molecular weight type I and type II enzymes and the form known as cytoplasmic PLA2 (cPLA2). cPLA2 is present in the cytosol of various cells and tissues including platelets, macrophages and monoblasts; and preferentially hydrolyzes the sn-2 position of phospholipid molecules, releasing free arachidonate.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :c-PLA2 (N499) polyclonal antibody detects endogenous levels of c-PLA2 protein.

BiowMW :~ 100 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phospholipase Signaling,G Protein-coupled Receptors Signaling to MAPK Erk,

Alternative Name :Cytosolic phospholipase A2; cPLA2; Phospholipase A2 group IVA; Phospholipase A2; Phosphatidylcholine 2-acylhydrolase Lysophospholipase; PLA2G4A; CPLA2; PLA2G4

Immunogen :Synthetic peptide, corresponding to amino acids 480-530 of Human c-PLA2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of c-PLA2 (N499) pAb at 1:500 dilution Lane1:THP-1 whole cell lysate(40ug) Lane2:MEF whole cell lysate(10ug) Lane3:H9C2 whole cell lysate(40ug) Lane4:K562 whole cell lysate(10ug) Lane5:Jurkat whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of c-PLA2 (N499) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Neuroprotection and underlying mechanisms of oxymatrine in cerebral ischemia of rats

JournalNeurological Research

ApplicationWB

IF2.401

SpeciesRat

PMID20626959
Ruscogenin suppresses mouse neutrophil activation: Involvement of protein kinase A pathway

JournalJournal Of Steroid Biochemistry And Molecular Biology

ApplicationWB

IF3.813

SpeciesMouse

PMID26134424
Corticotrophin-releasing factor participates in S1PR3-dependent cPLA2 expression and cell motility in vascular smooth muscle cells

JournalVascular Pharmacology

ApplicationWB

IF3.33

SpeciesRat

PMID25869501
UCN enhances TGF-beta-mediated mitoinhibition of VSMCs via counteracting TGF-beta-induced cPLA2 expression and activation

JournalInternational Journal Of Biochemistry & Cell Biology

ApplicationWB

IF3.144

SpeciesRat

PMID27702653
Urocortin participates in LPS-induced apoptosis of THP-1 macrophages via S1P-cPLA2 signaling pathway

JournalEuropean Journal Of Pharmacology

ApplicationWB

IF3.263

SpeciesHuman

PMID32949605