产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

Smad2 (P459) polyclonal antibody（KU）

货号: BS1425

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1425

Product Name :Smad2 (P459) polyclonal antibody（KU）

Swiss-Prot :Q15796

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP

Application_all :WB: 1:500~1:1000 IP: 1:50~1:200

Background :Members of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include SMAD1, 2, 3, 5, and 9; the common-mediator SMAD (co-SMAD), SMAD4; and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7. Activated type I receptors associate with specific R-SMADs and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-SMADs dissociate from the receptor and form a heteromeric complex with SMAD4, initiating translocation of the heteromeric SMAD complex to the nucleus. Once in the nucleus, SMADs recruit a variety of DNA binding proteins that function to regulate transcriptional activity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Smad2 (Ab-467) polyclonal antibody detects endogenous levels of Smad2 protein.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Tumor Angiogenesis,Contribution of Soluble Factors to EMT,hippo Signaling,TGFβ Smad signaling ,

Alternative Name :Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2

Immunogen :Synthetic peptide, corresponding to Human Smad2.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Smad2 (Ab-467) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(30ug) Lane2:Hela whole cell lysate(30ug) Lane3:PC12 whole cell lysate(30ug) Lane4:3T3-L1 whole cell lysate(30ug)

Epigenetic silencing of DACH1 induces loss of transforming growth factor‐β1 antiproliferative response in human hepatocellular carcinoma

JournalHepatology

ApplicationWB

IF14.971

SpeciesHuman

PMID23787902
Prostaglandin E2 promotes liver cancer cell growth by the upregulation of FUSE-binding protein 1 expression

JournalInternational Journal Of Oncology

ApplicationWB

IF3.571

SpeciesHuman

PMID23338277
A bilineage thermosensitive hydrogel system for stimulation of mesenchymal stem cell differentiation and enhancement of osteochondral regeneration

JournalComposites Part B-Engineering

ApplicationICC

IF9.078

SpeciesRabbit

PMID
The Astragaloside IV Derivative LS-102 Ameliorates Obesity-Related Nephropathy

JournalDrug Design Development and Therapy

ApplicationIHC,IF

IF4.162

SpeciesMouse

PMID35308255
Osteoclast-derived exosomal miR-212-3p suppressed the anabolism and accelerated the catabolism of chondrocytes in osteoarthritis by targeting TGF-β1/Smad2 signaling

JournalARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

ApplicationWB,IHC

IF3.9

SpeciesMouse,Human

PMID38000494