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Skp2 p45 (P399) polyclonal antibody

货号: BS1325

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1325

Product Name :Skp2 p45 (P399) polyclonal antibody

Swiss-Prot :Q13309

Host :Rabbit

Reactivity :Human

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The critical role that the family of regulatory proteins known as cyclins plays in eukaryotic cell cycle regulation is well established. The best characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of MPF (maturation promoting factor). Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin dependent kinase 2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DP-1 and E2F, and retinoblastoma protein p110. Two cyclin A-Cdk2 complex binding proteins, Skp1 p19 and Skp2 p45, have been described. Although the Skps (S phase kinase-associated proteins) associate with the active cyclin A-Cdk2 complex, they do not exhibit any regulatory effects on the complex. Abolition of Skp2 p45 function by either microinjection of anti-p45 antibodies or addition of antisense oligonucleotides prevents entry into S phase of both normal and transformed cells.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SKP2 p45 (P399) polyclonal antibody detects endogenous levels of SKP2 p45 protein.

BiowMW :~ 55 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :S-phase kinase-associated protein 2; Cyclin-A/CDK2-associated protein p45; F-box protein Skp2; F-box/LRR-repeat protein 1; p45skp2; SKP2; FBXL1

Immunogen :Synthetic peptide, corresponding to amino acids 369-419 of Human Skp2 p45.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SKP2 at 1:500 dilution Lane1:EC9706 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:A375 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Skp2 p45 (P399) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.