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Raf-1 (Q253) polyclonal antibody（KU）

货号: BS1307

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1307

Product Name :Raf-1 (Q253) polyclonal antibody（KU）

Swiss-Prot :P04049

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Raf1 (Q253) polyclonal antibody detects endogenous levels of Raf1 protein.

BiowMW :~ 73 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Inhibition of Apoptosis,G Protein-coupled Receptors Signaling to MAPK Erk,hippo Signaling,MAPK Erk in Growth and Differentiation Pathway,

Alternative Name :RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1; RAF1; RAF

Immunogen :Synthetic peptide, corresponding to amino acids 211-260 of Human Raf-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of c-Raf /Raf-1 (Q253) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:SGC-7901 whole cell lysate(40ug) Lane3:HCC827 whole cell lysate(40ug) Lane4:The Muscle tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of Raf-1 (Q253) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.