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PML (P39) polyclonal antibody（KU）

货号: BS1296

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1296

Product Name :PML (P39) polyclonal antibody（KU）

Swiss-Prot :P29590

Host :Rabbit

Reactivity :Human

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor α (RARα) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARα chimeric protein. Myeloid precursor cells expressing the PML/ RARα chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARα transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/ RARα also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PML (P39) polyclonal antibody detects endogenous levels of PML protein.

BiowMW :~ 98 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Protein PML; Promyelocytic leukemia protein; RING finger protein 71; Tripartite motif-containing protein 19; MYL; PP8675; RNF71; TRIM19

Immunogen :Synthetic peptide, corresponding to the N-terminal of Human PML.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PML (P39) pAb at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PML (P39) pAb in paraffin-embedded human tonsil carcinoma tissue at 1:50.

Suppression of RAD21 Induces Senescence of MDA‐MB‐231 Human Breast Cancer Cells Through RB1 Pathway Activation Via c‐Myc Downregulation

JournalJournal Of Cellular Biochemistry

ApplicationWB

IF4.237

SpeciesHuman

PMID