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PDPK1 (K235) polyclonal antibody(KU)

货号: BS1291
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS1291
Product Name :PDPK1 (K235) polyclonal antibody(KU)
Swiss-Prot :O15530
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB IHC
Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200
Background :PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :PDPK1 (K235) polyclonal antibody detects endogenous levels of PDPK1 protein.
BiowMW :~ 63 kDa
Note :For research use only, not for use in diagnostic procedure.
Pathway :Phosphoinosotode Signaling,PI3K AKT signaling,Protein Kinase C Signaling,Translational Contral elF4 and p70 S6 Kinase,Inhibition of Apoptosis,Insulin &Glucose Signaling,MTOR signaling,
Alternative Name :3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1
Immunogen :Synthetic peptide, corresponding to amino acids 211-260 of Human PDPK1.
Conjugate :Unconjugated
Modification :Unmodification
Western blot (WB) analysis of PDPK1 (K235) Antibody at 1:1000 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug)
Immunohistochemistry (IHC) analyzes of PDPK1 (K235) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
  • LncRNA KTN1-AS1 promotes the progression of non-small cell lung cancer via sponging of miR-130a-5p and activation of PDPK1
    JournalOncogene
    ApplicationWB
    IF7.971
    SpeciesHuman,Mouse
  • Genetic inhibition of PDK1 robustly reduces plaque deposition and ameliorates gliosis in the 5×FAD mouse model of Alzheimer's disease
    JournalNEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
    ApplicationIHC,IF
    IF6.25
    SpeciesMouse
  • CcpA-Knockout Staphylococcus aureus Induces Abnormal Metabolic Phenotype via the Activation of Hepatic STAT5/PDK4 Signaling in Diabetic Mice
    JournalPathogens
    ApplicationWB
    IF3.7
    SpeciesHuman,Mouse
    PMID

关注我们

400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com