Product Name :PDPK1 (K235) polyclonal antibody(KU)
Reactivity :Human,Mouse,Rat
Application_all :WB: 1:500~1:1000
IHC: 1:50~1:200
Background :PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of hosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :PDPK1 (K235) polyclonal antibody detects endogenous levels of PDPK1 protein.
Note :For research use only, not for use in diagnostic procedure.
Pathway :Phosphoinosotode Signaling,PI3K AKT signaling,Protein Kinase C Signaling,Translational Contral elF4 and p70 S6 Kinase,Inhibition of Apoptosis,Insulin &Glucose Signaling,MTOR signaling,
Alternative Name :3-phosphoinositide-dependent protein kinase 1; hPDK1; PDPK1; PDK1
Immunogen :Synthetic peptide, corresponding to amino acids 211-260 of Human PDPK1.
Modification :Unmodification