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PCNA (I88) polyclonal antibody（KU）

货号: BS1289

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1289

Product Name :PCNA (I88) polyclonal antibody（KU）

Swiss-Prot :P12004

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication, and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli, however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms, one involved in ongoing DNA replication. which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PCNA (I88) polyclonal antibody detects endogenous levels of PCNA protein.

BiowMW :~ 34 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Proliferating cell nuclear antigen; Cyclin

Immunogen :Synthetic peptide, corresponding to amino acids 51-100 of Human PCNA.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of PCNA (I88) polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of PCNA (I88) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of Hela cell lysates using PCNA pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using PCNA pAb.

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