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Patched (A2) polyclonal antibody（KU）

货号: BS1286

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1286

Product Name :Patched (A2) polyclonal antibody（KU）

Swiss-Prot :Q13635

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Patched (A2) polyclonal antibody detects endogenous levels of Patched protein.

BiowMW :~ 160 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hedgehog Signaling,

Alternative Name :Protein patched homolog 1; PTC; PTC1; PTCH1; PTCH

Immunogen :Synthetic peptide, corresponding to the N-terminal of Human Patched.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Patched (A2) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:The Testis tissue lysate of Mouse(40ug) Lane6:The Testis tissue lysate of Rat(40ug)

Immunohistochemistry (IHC) analyzes of Patched (A2) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Overexpression of Hedgehog signaling molecules and its involvement in triple-negative breast cancer

JournalOncology Letters

ApplicationIHC

IF2.311

SpeciesHuman

PMID
Dihydrotanshinone I Inhibits Pancreatic Cancer Progression via Hedgehog/ Gli Signal Pathway

JournalCURRENT CANCER DRUG TARGETS

ApplicationWB

IF3

SpeciesHuman，Mouse

PMID37018533