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NSE/Enolase (R400) polyclonal antibody（KU）

货号: BS1259

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1259

Product Name :NSE/Enolase (R400) polyclonal antibody（KU）

Swiss-Prot :P09104

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits (alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE (neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include pediatric euroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NSE/ENO2 (R400) polyclonal antibody detects endogenous levels of NSE/ENO2 protein.

BiowMW :~ 47 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Hypoxia Signaling,Warburg Effect,

Alternative Name :Gamma-enolase; Gamma-enolase; Enolase 2; Neural enolase; Neural enolase; NSE; ENO2

Immunogen :Synthetic peptide, corresponding to amino acids 361-410 of Human NSE/Enolase.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NSE/Enolase (R400) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:The brain tissue lysate of Mouse(40ug) Lane3:The brain tissue lysate of Rat(40ug) Lane4:The lung tissue lysate of Mouse(40ug)

Immunohistochemistry (IHC) analyzes of NSE/Enolase (R400) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

The mitochondria/caspase-dependent apoptotic pathway plays a role in the positive effects of a power frequency electromagnetic field on Alzheimer’s disease neuronal model

JournalJournal Of Chemical Neuroanatomy

ApplicationIHC

IF3.052

SpeciesRat

PMID32918997