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NFkB-p65 (G530) polyclonal antibody（KU）

货号: BS1257

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1257

Product Name :NFkB-p65 (G530) polyclonal antibody（KU）

Swiss-Prot :Q04206

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB IP IF

Application_all :WB: 1:500~1:1000 IF: 1:50~1:200 IP: 1:50~1:200

Background :p65 is a subunit of the nuclear factor kappa B. The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory prd in many tissues. A high proportion of spontaneous NIH/3T3 transformants overexpress c-Met and by transfection analysis the c-Met proto-oncogene has been shown to exhibittransforming activity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NFκB-p65 (G530) polyclonal antibody detects endogenous levels of NFκB-p65 protein.

BiowMW :~ 65 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,NF-kB Signaling,Immune Cell Signaling,Signaling Pathways Activating p38 MAP KinaseSTING Signaling,T cell receptor signaling,Inhibition of Apoptosis,Contribution of Soluble Factors to EMT,Toll-like Receptor signaling,

Alternative Name :p65; p65 NF kappaB; p65 NFkB; RELA; NFKB P65; Transcription Factor p65; Nuclear factor NF-kappa-B p65 subunit; NFkB-p65; NFKB3

Immunogen :Synthetic peptide, corresponding to Human NFkB-p65.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NfkB-p65 pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug) Lane5:CT-26 whole cell lysate(40ug)

Immunoprecipitation of MCF-7 cell lysates using NFkB-p65 (G530) pAb (Sepharose Bead Conjugate)#BD0048 (lane 3 and lane 4) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1 and lane 2) .Lane 5 is 30% input. The western blot was probed using NFkB-p65 (G530) pAb #BS1257.

Immunofluorescence analysis of C6 cells using NFkB-p65 (G530) antibody at dilution of 1:50.

Western blot (WB) analysis of NfkB-p65 pAb at 1:1000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:NfkB-p65 knockout A549 whole cell lysate(40ug)

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