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NFkB-p105 (A901) polyclonal antibody（KU）

货号: BS1251

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1251

Product Name :NFkB-p105 (A901) polyclonal antibody（KU）

Swiss-Prot :P19838

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :Proteins encoded by the v-Rel viral oncogene and its cellular homolog, c-Rel, are members of a family of transcription factors that include the two subunits of the transcription factor NFκB (p50 and p65) and the Drosophila maternal morphogen, dorsal. The DNA binding activity of NFκB is activated and NFκB is subsequently transported from the cytoplasm to the nucleus in cells exposed to mitogens or growth factors. cDNAs encoding precursors for two distinct proteins of the same size have been described, designated p105 and p100. The p105 precursor contains p50 at its N-terminus and a C-terminal region that when expressed as a separate molecule, designated pdI, binds to p50 and regulates its activity

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NFkB-p105 (A901) polyclonal antibody detects endogenous levels of NFκB-p105 protein.This antibody does not recognize NFkB-p50 subunit.

BiowMW :~ 105 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :NF-kB Signaling,STING Signaling,Inhibition of Apoptosis,

Alternative Name :Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light; polypeptide gene enhancer in B-cells 1; NFKB1; NFkB p105;

Immunogen :Synthetic peptide, corresponding to amino acids 920-970 of Human NFkB-p105.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of NFkB-p105 (A901) polyclonal antibody at 1:500 dilution Lane1:SK-OVCAR3 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:L02 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of NFkB-P105 (A901) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of L02 cell lysate using NFkB-p105 (A901) pAb (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using NFkB-p105 (A901) pAb #BS1251.“↑”(supernatant); “↓”(deposition)