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MMP-2 (L638) polyclonal antibody

货号: BS1236

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1236

Product Name :MMP-2 (L638) polyclonal antibody

Swiss-Prot :P08253

Host :Rabbit

Reactivity :Human,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MMP-2 (L638) polyclonal antibody detects endogenous levels of MMP-2 protein.

BiowMW :~ 65, 24 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Contribution of ECM and Cytoskeletal Factors to EMT,

Alternative Name :72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2; TBE-1; TBE1; PEX; MMP2; CLG4A

Immunogen :Synthetic peptide, corresponding to Human MMP-2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MMP-2 (L638) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate LaneB:HuT78 whole cell lysate LaneC:H1792 whole cell lysate LaneD:The Brain tissue lysate of Mouse(40ug) LaneE:The Lung tissue lysate of Mouse(40ug) LaneF:The Brain tissue lysate of Rat(40ug) LaneG:The Lung tissue lysate of Rat(40ug)

Immunofluorescence analysis of Hela cells using MMP-2 antibody at dilution of 1:50.

Immunohistochemistry of paraffin-embedded human lung using MMP-2 antibody at dilution of 1:50.

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