产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

MMP-1 (H440) polyclonal antibody（KU）

货号: BS1229

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1229

Product Name :MMP-1 (H440) polyclonal antibody（KU）

Swiss-Prot :P03956

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92 kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. MMP-1 is downregulated by p53, and abnormality of p53 expression may contribute to joint degradation in rheumatoid arthritis by regulating MMP-1 expression

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MMP-1 (H440) polyclonal antibody detects endogenous levels of MMP-1 protein.

BiowMW :~ 54 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Interstitial collagenase; Fibroblast collagenase; Matrix metalloproteinase-1; MMP-1; 22 kDa interstitial collagenase; 27 kDa interstitial collagenase; MMP1; CLG

Immunogen :Synthetic peptide, corresponding to amino acids 411-460 of Human MMP-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MMP-1 (H440) pAb at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:MCF-7 whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug) Lane5:HepG2 whole cell lysate(40ug)

Western blot (WB) analysis of MMP-1 (H440) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(30ug) Lane2:The kidney tissue lysate of Mouse(40ug) Lane3:The kidney tissue lysate of Rat(15ug) Lane4:L02 whole cell lysate(30ug)

Trichostatin A increases the TIMP-1/MMP ratio to protect against osteoarthritis in an animal model of the disease

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesRat

PMID27431944
Protective effect of glucosamine cyclohexyl ester on osteoarthritis in rat via targeting expressions of matrix metalloproteinase and tissue inhibitor of metalloproteinases-1

JournalTropical Journal Of Pharmaceutical Research

ApplicationWB

IF0.504

SpeciesRat

PMID
HtrA1 upregulates the expression of ADAMTS-5 in HNPCs via the ERK/NF-κB/JNK signaling pathway

JournalAmerican Journal of Translational Research

ApplicationWB

IF4.06

SpeciesHuman

PMID31497227
Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB signalling pathway

JournalMolecular And Cellular Endocrinology

ApplicationWB

IF3.871

SpeciesRat

PMID32877753
Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation

JournalCancer Cell International

ApplicationWB

IF3.439

SpeciesHuman

PMID31572058