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MDM2 (F414) polyclonal antibody（KU）

货号: BS1223

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1223

Product Name :MDM2 (F414) polyclonal antibody（KU）

Swiss-Prot :Q00987

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. These findings argue that MDM2 overexpression represents at least one mechanism by which p53 function can be abrogated during tumorigenesis.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MDM2 (F414) polyclonal antibody detects endogenous levels of MDM2 protein.

BiowMW :~ 95 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Examples of Crosstalk Between Post-translational Modifications,Cell Cycle G2 M DNA Damage Signaling Pathway,

Alternative Name :E3 ubiquitin-protein ligase Mdm2; Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2; MDM2

Immunogen :Synthetic peptide, corresponding to amino acids 385-430 of Human MDM2.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MDM2 pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:HepG2 whole cell lysate(40ug) Lane5:Pancl whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of MDM2 (F414) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

The E3 ubiquitin protein ligase MDM2 dictates all-trans retinoic acid-induced osteoblastic differentiation of osteosarcoma cells by modulating the degradation of RARα

JournalOncogene

ApplicationIHC

IF9.867

SpeciesHuman

PMID26776160
Tris (2-chloroethyl) phosphate induces senescence-like phenotype of hepatocytes via the p21Waf1/Cip1-Rb pathway in a p53-independent manner

JournalEnvironmental Toxicology And Pharmacology

ApplicationWB

IF4.86

SpeciesHuman

PMID28886428
PCAF Accelerates Vascular Senescence via the Hippo Signaling Pathway

JournalOxidative Medicine and Cellular Longevity

ApplicationWB

IF7.31

SpeciesHuman

PMID36246398
Etoposide-induced SENP8 confers a feed-back drug resistance on acute lymphoblastic leukemia cells

JournalBiochemistry and Biophysics Reports

ApplicationWB

IF2.7

SpeciesHuman

PMID38314144