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Mcl-1 (S121) polyclonal antibody （KU）

货号: BS1220

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1220

Product Name :Mcl-1 (S121) polyclonal antibody （KU）

Swiss-Prot :Q07820

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IP

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IP: 1:50~1:200

Background :B cell CLL/lymphoma-2 (Bcl-2) blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. Myeloid cell leukemia-1 (Mcl-1) shares sequence homology with Bcl-2 and further resembles Bcl-2 in that its expression promotes cell viability. p53 and Mcl-1 demonstrate opposing effects on mitochondrial apoptosis by mediating Bcl-2 antagonist killer (Bak) activity. Mcl-1 is an important and specific regulator that is necessary for the homeostasis of early hematopoietic progenitors. Glycogen synthase kinase-3 (GSK-3) controls Mcl-1 stability, which has an effect on the regulation of apoptosis by growth factors PI 3-kinase and Akt. Mice with a deficiency of the Mcl-1 protein show a significant reduction in B and T lymphocytes similar to the effects observed in IL-7- or IL-7R-deficient mice.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MCL1 (S121) polyclonal antibody detects endogenous levels of MCL1 protein.

BiowMW :~ 37 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Mitochondrial Control of Apoptosis,

Alternative Name :Induced myeloid leukemia cell differentiation protein Mcl-1; Bcl-2-like protein 3; Bcl2-L-3; Bcl-2-related protein EAT/mcl1; mcl1/EAT; MCL1; BCL2L3

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human Mcl-1.

Conjugate :Unconjugated

Modification :Unmodified

Western blot (WB) analysis of Mcl-1 (S121) polyclonal antibody at 1:500 dilution Lane1:HCT116 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of Mcl-1 (S121) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Immunoprecipitation of HEK293T cell lysate using Mcl-1 (S121) polyclonal antibody (Sepharose Bead Conjugate) #BD0048(lane 2 and lane 3).Lane 1 is 30% input.The western blot was probed using Mcl-1 (S121).“↑”（supernatant）;“↓(deposition）

Probing the difference between BH3 groove of Mcl-1 and Bcl-2 protein: Implications for dual inhibitors design

JournalEuropean Journal Of Medicinal Chemistry

ApplicationIF

IF6.514

Species

PMID21684048
A novel BH3 mimetic S1 potently induces Bax/Bak‐dependent apoptosis by targeting both Bcl‐2 and Mcl‐1

JournalInternational Journal Of Cancer

ApplicationWB

IF4.982

SpeciesHuman,Mouse

PMID
Pan-BH3 Mimetic S1 Exhibits Broad-Spectrum Antitumour Effects by Cooperation between Bax and Bak

JournalBasic & Clinical Pharmacology & Toxicology

ApplicationWB

IF4.08

SpeciesHuamn

PMID23557083
Sorafenib induces growth inhibition and apoptosis in human synovial sarcoma cells via inhibiting the RAF/MEK/ERK signaling pathway

JournalCancer Biology & Therapy

ApplicationWB

IF3.659

SpeciesHuman

PMID19633425
Sorafenib induces growth inhibition and apoptosis of human chondrosarcoma cells by blocking the RAF/ERK/MEK pathway

JournalJournal Of Surgical Oncology

ApplicationWB

IF2.771

SpeciesHuman

PMID