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LIMK1/2 (D402) polyclonal antibody

货号: BS1215

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1215

Product Name :LIMK1/2 (D402) polyclonal antibody

Swiss-Prot :P53667/P53671

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :LIMK1/2 (D402) polyclonal antibody detects endogenous levels of LIMK1 and LIMK 2 protein.

BiowMW :~ 72 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Actin Dynamics,Regulation of Microtubule Dynamics,

Alternative Name :LIM domain kinase 1; LIMK-1; LIMK1; LIMK; LIM domain kinase 2; LIMK 2; LIMK2

Immunogen :Synthetic peptide, corresponding to amino acids 380-420 of Human LIMK1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of LIMK1/2 (D402) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:Panc1 whole cell lysate(40ug) Lane3:A549 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:AML-12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of LIMK1/2 (D402) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.