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CHOP (K121) polyclonal antibody

货号: BS1136

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1136

Product Name :CHOP (K121) polyclonal antibody

Swiss-Prot :P35638

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :GADD153 is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP GADD153 dimer gains binding activity to other non classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly unregulated during times of cellular/ER stress. Examples of GADD153 inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :CHOP (K121) polyclonal antibody detects endogenous levels of CHOP protein.

BiowMW :~ 19,27 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Signaling Pathways Activating p38 MAP Kinase,

Alternative Name :DNA damage-inducible transcript 3 protein; DDIT-3; C/EBP zeta; C/EBP-homologous protein; CHOP; C/EBP-homologous protein 10; CHOP-10; CCAAT/enhancer-binding protein homologous protein; Growth arrest and DNA damage-inducible protein GADD153; DDIT3; CHOP; CHOP10; GADD153

Immunogen :Synthetic peptide, corresponding to amino acids 100-150 of Human CHOP.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CHOP (K121) pAb at 1:500 dilution Lane1:A375 whole cell lysate(40ug) Lane2:HCT116 whole cell lysate(40ug) Lane3:The Kidney tissue lysate of Mouse(40ug) Lane4:PC12 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of CHOP (K121) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

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