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CUL1 (Y761) polyclonal antibody（KU）

货号: BS1081

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS1081

Product Name :CUL1 (Y761) polyclonal antibody（KU）

Swiss-Prot :Q13616

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC IF

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Cullin 1 (Y761) polyclonal antibody detects endogenous levels of Cullin 1 protein.

BiowMW :~ 90 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CUL 1; CUL-1; CUL1; CUL1; Cullin-1; Cullin1; MGC149834; MGC149835;

Immunogen :Synthetic peptide, corresponding to amino acids 728-780 of Human CUL-1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of CUL1 (Y761) pAb at 1:500 dilution Lane1:The Brain tissue lysate of Rat(40ug) Lane2:The Lung tissue lysate of Rat(40ug) Lane3:The Lung tissue lysate of Mouse(40ug) Lane4:A549 whole cell lysate(40ug) Lane5:HCC827 whole cell lysate(40ug)

Immunohistochemistry (IHC) analyzes of CUL-1 (Y761) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.