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Chk1 (K274) polyclonal antibody（KU）

货号: BS1051

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS1051

Product Name :Chk1 (K274) polyclonal antibody（KU）

Swiss-Prot :O14757

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due tophosphorylation by Chk1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Chk1 (K274) polyclonal antibody detects endogenous levels of Chk1 protein.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Cell Cycle Control G1 S Checkpoint,Cell Cycle G2 M DNA Damage Signaling Pathway,Epigenetic Writers and Erasers of Histone H3,

Alternative Name :Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1; CHEK1; CHK1

Immunogen :Synthetic peptide, corresponding to amino acids 251-300 of Human Chk1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Chk1 (K274) polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:SGC7901 whole cell lysate(40ug) Lane3:HCT116 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug)