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Actin (E361) polyclonal antibody（KU）

货号: BS1002

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS1002

Product Name :Actin (E361) polyclonal antibody（KU）

Swiss-Prot :P60709/Q9BYX7/P63261

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IHC

Application_all :WB: 1:500~1:1000 IHC: 1:50~1:200

Background :All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Alpha Actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion, Rac regulates Actin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Actin (E361) polyclonal antibody detects endogenous levels of Actin protein.

BiowMW :~ 45 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ACTB; Beta-actin; Actin; cytoplasmic 1; β-actin; β actin; βactin; actin-β; actin β, POTEKP, Kappa-actin, ACTBL3, Gamma-actin, ACTG1, ACTG

Immunogen :Synthetic peptide, corresponding to amino acids C-terminus of Human Actin.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of Actin (E361) polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate(40μg) Lane2:HEK293T whole cell lysate(40μg) Lane3:NIH-3T3 whole cell lysate(40μg) Lane4:RAW264.7 whole cell lysate(40μg) Lane5:PC12 whole cell lysate(40μg)

Immunohistochemistry (IHC) analyzes of Actin (E361) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and cytoskeleton staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Immunohistochemistry (IHC) analyzes of Actin (E361) pAb in paraffin-embedded human liver carcinoma tissue at 1:50.showing cytoplasmic and cytoskeleton staining. Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

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