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MAP1LC3B polyclonal antibody（KU）

货号: AP6043

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP6043

Product Name :MAP1LC3B polyclonal antibody（KU）

Swiss-Prot :Q9GZQ8

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IP IF

Application_all :WB: 1:500~1:2000 IP: 1:50~1:200 IF: 1:100~1:500

Background :MAP1LC3B, a homolog of Apg8p, is essential for autophagy and associated to the autophagosome membranes after processing. Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MAP1LC3B polyclonal antibody detects endogenous levels of MAP1LC3B protein.

BiowMW :~ 16 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling

Alternative Name :Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II

Immunogen :Synthetic peptide, corresponding to Human MAP1LC3B.

Conjugate :Unconjugated

Modification :Unmodified

Immunofluorescence analysis of MCF-7 cells using MAP1LC3B pAb at dilution of 1:200 (40x lens).

Immunoprecipitation of Hela cell lysates using MAP1LC3B pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4) .Lane 1 is 30% input. The western blot was probed using MAP1LC3B pAb.

HucMSC exosome-delivered 14-3-3ζ alleviates ultraviolet radiation-induced photodamage via SIRT1 pathway modulation

JournalAging-US

ApplicationIF

IF4.831

SpeciesHuman

PMID33882455
FOXO3 Inhibits Human Gastric Adenocarcinoma (AGS) Cell Growth by Promoting Autophagy in an Acidic Microenvironment

JournalCellular Physiology And Biochemistry

ApplicationWB

IF0

SpeciesHuman

PMID30138933