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LC3 (phospho-Thr50) polyclonal antibody（KU）

货号: AP4013

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :AP4013

Product Name :LC3 (phospho-Thr50) polyclonal antibody（KU）

Swiss-Prot :Q9GZQ8/Q9H492/Q9BXW4

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Two forms of LC3β , the cytosolic LC3-I and the membrane-bound LC3-II, are produced post-translationally. LC3-I is formed by the removal of the C-terminal 22 amino acids from newly synthesized LC3β , followed by the conversion of a fraction of LC3-I into LC3-II. LC3 enhances fibronectin mRNA translation in ductus arteriosus cells through association with 60S ribosomes and binding to an AU-rich element in the 3’ untranslated region of fibronectin mRNA. This facilitates sorting of fibronectin mRNA onto rough endoplasmic reticulum and translation. MAP LC3β may also be involved in formation of autophagosomal vacuoles. It is expressed primarily in heart, testis, brain and skeletal muscle.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :LC3 (phospho-Thr50) polyclonal antibody detects endogenous levels of LC3A and LC3B protein only when phosphorylated at Thr50. This antibody also recognizes LC3C when phosphorylated at the corresponding residues.

BiowMW :~ 15 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Phosphoinosotode Signaling,Autophagy Signaling,

Alternative Name :Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3; LC3-I; LC3-II; LC3I; LC3II; MAP1LC3A

Immunogen :Synthetic phosphopeptide derived from human LC3 around the phosphorylation site of Threonine 50.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of LC3 (phospho-Thr50) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:L02 treated with PBS(1×PBS,PH7.4) for 1 hour whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:HEK293T treated with Etoposide(20ng/ml) for 30 minutes whole cell lysate(40ug) Lane5:HEK293T treated with Etoposide(20ng/ml) for 6 hours whole cell lysate(40ug)

Western blot (WB) analysis of LC3 (Phospho-Thr50) pAb at 1:500 dilution Lane1:HEK293T transfected with pEGFP-C3-LC3A whole cell lysate(10ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:GFP protein(100ng) Lane4:HEK293T transfected with pEGFP-C3-LC3B whole cell lysate(10ug) Lane5:HEK293T transfected with pEGFP-C3-LC3B whole cell lysate(10ug) Lane6:HEK293T whole cell lysate(40ug) Lane7:HEK293T transfected with pEGFP-C3-LC3A whole cell lysate(10ug)