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MLST8 polyclonal antibody（KU）

货号: AP1000

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP1000

Product Name :MLST8 polyclonal antibody（KU）

Swiss-Prot :Q9BVC4

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB

Application_all :WB: 1:500~1:1000

Background :Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E).

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :MLST8 polyclonal antibody detects endogenous levels of MLST8 protein.

BiowMW :~ 36 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Glutamine Metabolism,MTOR signaling,

Alternative Name :G protein beta subunit like; G protein beta subunit-like; Gable; GBL; GBL protein; LST8; Mammalian lethal with SEC13 protein 8; MGC111011; mLST8; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit LST8;

Immunogen :Synthetic peptide, corresponding Human MLST8.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of MLST8 polyclonal antibody at 1:500 dilution Lane1:The Heart tissue lysate of Mouse(40ug) Lane2:The Heart tissue lysate of Rat(40ug) Lane3:HCC827 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug) Lane5:HEK293T whole cell lysate(40ug)

Western blot analysis of extracts from HEK293T cells(Lane 3), HEK293T cells transfected with control shRNA for72h (-) (Lane 2) or MLST8 shRNA (+)(Lane 1).MLST8 was detected using MLST8 pAb #AP1000.The MLST8 Antibody confirms silencing of MLST8 expression.