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ATG9B (G883) polyclonal antibody（KU）

货号: AP0781

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0781

Product Name :ATG9B (G883) polyclonal antibody（KU）

Swiss-Prot :Q674R7

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB IP

Application_all :WB: 1:5000~1:10000 IP: 1:50~1:200

Background :Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion. In addition to autophagy, also plays a role in necrotic cell death.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATG9B (G883) polyclonal antibody detects endogenous levels of ATG9B protein.

BiowMW :~ 95 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Autophagy-related protein 9B; APG9-like 2; Nitric oxide synthase 3-overlapping antisense gene protein; Protein sONE; ATG9B; APG9L2

Immunogen :Synthetic peptide, corresponding to Human ATG9B.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATG9B (G883) polyclonal antibody at 1:5000 dilution Lane1:C6 whole cell lysate(40ug) Lane2:BV2 whole cell lysate(40ug) Lane3:The Brain tissue lysate of Rat(40ug) Lane4:The Brain tissue lysate of Mouse(40ug) Lane5:MCF-7 whole cell lysate(40ug) Lane6:HepG2 whole cell lysate(40ug) Lane7:Jurkat whole cell lysate(40ug)

Immunoprecipitation of HEK293T cell lysates using ATG9B (G883) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 1) .Lane 4 is 30% input. The western blot was probed using ATG9B (G883) pAb #AP0781.