产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

ATG4D (G30) polyclonal antibody

货号: AP0777

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0777

Product Name :ATG4D (G30) polyclonal antibody

Swiss-Prot :Q86TL0

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :Autophagy, a process that results in the lysosomal-dependent degradation of cytosolic compartments, is carried out by the autophagosome, which is a double-membrane vesicle whose formation is catalyzed by several autophagyrelated gene (Atg) proteins. Atg4D (autophagy-related gene 4D), also known as APG4D or AUTL4, is a 474 amino acid protein that localizes to the cytoplasm and belongs to the C-54 family of cysteine proteases. Expressed predominately in skeletal muscle, but also present in testis, Atg4D functions as a cysteine protease that is required for autophagy and functions to specifically cleave the C-terminal region of target proteins, thereby allowing the target proteins to bind to autophagosomes. The enzymatic activity of Atg4D is inhibited by N-ethylmaleimide, a thiol reactive compound that is capable of modifying cystine residues in proteins and peptides.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ATG4D(G30) polyclonal antibody detects endogenous levels of ATG4D protein.

BiowMW :~ 52 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Cysteine protease ATG4D; AUT-like 4 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 4; Autophagin-4; Autophagy-related protein 4 homolog D; HsAPG4D; Cysteine protease ATG4D, mitochondrial

Immunogen :Synthetic peptide, corresponding to Human ATG4D.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of ATG4D (G30) polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)