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SQSTM1 (R415) polyclonal antibody（KU）

货号: AP0772

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :AP0772

Product Name :SQSTM1 (R415) polyclonal antibody（KU）

Swiss-Prot :Q13501

Host :Rabbit

Reactivity :Human,Mouse

Applications :WB IF IHC

Application_all :WB: 1:5000~1:10000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :Sequestosome 1 (SQSTM1, p62) is a ubiquitin binding protein involved in cell signaling, oxidative stress, and autophagy. It was first identified as a protein that binds to the SH2 domain of p56Lck and independently found to interact with PKCζ. SQSTM1 was subsequently found to interact with ubiquitin, providing a scaffold for several signaling proteins and triggering degradation of proteins through the proteasome or lysosome. Interaction between SQSTM1 and TRAF6 leads to the K63-linked polyubiquitination of TRAF6 and subsequent activation of the NF-κB pathway. Protein aggregates formed by SQSTM1 can be degraded by the autophagosome. SQSTM1 binds autophagosomal membrane protein LC3/Atg8, bringing SQSTM1-containing protein aggregates to the autophagosome. Lysosomal degradation of autophagosomes leads to a decrease in SQSTM1 levels during autophagy; conversely, autophagy inhibitors stabilize SQSTM1 levels. Studies have demonstrated a link between SQSTM1 and oxidative stress. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. Thus, accumulation of SQSTM1 can lead to an increase in NRF2 activity.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :SQSTM1 (R415) polyclonal antibody detects endogenous levels of SQSTM1 protein.

BiowMW :~ 56 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Autophagy Signaling,

Alternative Name :Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62; SQSTM1; ORCA; OSIL

Immunogen :Synthetic peptide, corresponding to Human SQSTM1.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of SQSTM1 (R415) polyclonal antibody at 1:5000 dilution Lane1:EC9706 whole cell lysate(40ug) Lane2:SHSY5Y whole cell lysate(40ug Lane3:CT-26 whole cell lysate(40ug)

Immunofluorescence analysis of U-87MG cells using SQSTM1 (R415) antibody at dilution of 1:50.