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PPARG (Phospho-T166) polyclonal antibody（KU）

货号: AP0685

价格：￥ 1350

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0685

Product Name :PPARG (Phospho-T166) polyclonal antibody（KU）

Swiss-Prot :P37231

Host :Rabbit

Reactivity :Human

Applications :WB

Application_all :WB: 1:1000~1:2000

Background :Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PPARG (Phospho-T166) polyclonal antibody detects endogenous levels of PPARG protein only when phosphorylated at Thr166.

BiowMW :~ 60 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Nuclear Receptor Signaling,MTOR signaling,

Alternative Name :Peroxisome proliferator-activated receptor gamma; Short name=PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3; PPAR γ; PPARγ

Immunogen :Synthetic phosphopeptide derived from human PPARG around the phosphorylation site of Threonine 166.

Conjugate :Unconjugated

Modification :Phosphorylation

Western blot (WB) analysis of PPARG (Phospho-T166) polyclonal antibody at 1:1000 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:MCF-7 treated with TNF-alpha(20 ng/ml,10 minutes) and Calyculin A(100 nM,10 minutes) whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)

Tumor-secreted GRP78 induces M2 polarization of macrophages by promoting lipid catabolism

JournalCELLULAR SIGNALLING

ApplicationWB

IF4.85

SpeciesHuman

PMID37207940