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PPAR-gama polyclonal antibody（KU）

货号: AP0659

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0659

Product Name :PPAR-gama polyclonal antibody（KU）

Swiss-Prot :P37231

Host :Rabbit

Reactivity :Human,Rat,Mouse

Applications :WB IF

Application_all :WB: 1:1000~1:2000 IF: 1:50~1:200

Background :Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :PPAR-gama polyclonal antibody detects endogenous levels of PPAR-gama protein.

BiowMW :~ 50 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Nuclear Receptor Signaling,MTOR signaling,

Alternative Name :Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3

Immunogen :Synthetic peptide, corresponding to Human PPAR-gama.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of PPAR-gama polyclonal antibody at 1:1000 dilution Lane1:The Liver tissue lysate of Mouse(30ug) Lane2:The Kidney tissue lysate of Rat(30ug) Lane3:THP-1 whole cell lysate(30ug) Lane4:MCF-7 whole cell lysate(30ug) Lane5:PC12 whole cell lysate(30ug) Lane6:CT26 whole cell lysate(30ug)

Immunofluorescence analysis of MCF-7 cells using PPAR-gama antibody at dilution of 1:50.