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IkB-α (Y42) polyclonal antibody

货号: AP0643

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :AP0643

Product Name :IkB-α (Y42) polyclonal antibody

Swiss-Prot :P25963

Host :Rabbit

Reactivity :Human

Applications :WB IP IF IHC

Application_all :WB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200

Background :The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :IkB-α(T42) polyclonal antibody detects endogenous levels of IkB-α protein.

BiowMW :~ 39 kDa

Note :For research use only, not for use in diagnostic procedure.

Pathway :Regulation of Apoptosis,Death Receptor Signaling,MAPK Erk in Growth and Differentiation Pathway(,Inhibition of Apoptosis,NF-kB Signaling,Phospholipase Signaling,T cell receptor signaling,Toll-like Receptor signaling,

Alternative Name :NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha;IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD3; NFKBI

Immunogen :Synthetic peptide, corresponding to Human IkB-α.

Conjugate :Unconjugated

Modification :Unmodification

Western blot (WB) analysis of IkB-α (Y42) polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HepG2 whole cell lysate(40ug)

Immunoprecipitation of HepG2 cell lysates using IkB-α(Y42) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using IkB-α(Y42) pAb #AP0643.

Immunofluorescence analysis of A549 cells using IkB-α (Y42) antibody at dilution of 1:50.

Gualou Xiebai Decoction ameliorates cardiorenal syndrome type II by regulation of PI3K/AKT/NF-κB signalling pathway

JournalPHYTOMEDICINE

ApplicationWB

IF7.9

SpeciesRat

PMID37976694